%0 Journal Article
%T Purification of Vancomycin by Using Magnetic Affinity Technique<br>万古霉素的磁性亲和吸附分离
%A L A Guo
%A B Q Zhu
%A D J Chen
%A <br>郭立安
%A 朱宝泉
%A 陈代杰
%J 生物工程学报
%D 2001
%I 
%X A new kind of magnetic affinity microspheres (MAMS), whose ligand is D-Ala-D-Ala, was prepared using agarose as matrix. By using this new MAMS vancomycin was purified directly from crude fermentation liquor with only one step. The purity and the mass recovery of vancomycin measured by reverse-phase HPLC were 97% and 87%, respectively. The characteristic of this method was simpler, faster, cheaper and more effective than that of currently used ones.
%K vancomycin
%K purification
%K magnetic separation
%K affinity chromatography<br>万古霉素
%K 纯化
%K 磁性分离
%K 亲和色谱
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=2BC2EA9BE2EC47FD&yid=14E7EF987E4155E6&vid=BCA2697F357F2001&iid=94C357A881DFC066&sid=E04FC1B5BC47587B&eid=CD26609C367AC9C8&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=6&reference_num=12