%0 Journal Article
%T Cloning of VEGF Receptor KDR and its Expression in Insect Cells<br>VEGF受体KDR胞外区基因的克隆及其在昆虫细胞中的表达
%A ZENG Ge
%A |Fei
%A ZHANG Zhi
%A |Qing
%A ZHANG Li
%A |Guo
%A CHEN Ai
%A |Jun
%A YAO Li
%A |Hong
%A HOU Yun
%A |De
%A <br>曾革非
%A 张智清
%A 张立国
%A 陈爱君
%A 姚立红
%A 侯云德
%J 生物工程学报
%D 2001
%I 
%X The cDNA fragment of the first 3 loops of VEGF receptor, KDR, was cloned by PCR and inserted into a baculovirus expression plasmid pF AST B AC I. The competent \%E.coli\% DH10BAC cell, which contain another plasmid with baculovirus genome in it, was transformed with pF AST B AC I\|KDR\-\{n3\}. Homologous recombination in the prokaryotic cells resulted in a recombinant plasmid containing KDR\-\{n3\} in baculovirus genome. Transfection of the insect cell SF\|9 with above plasmid genatated a recombinant baculorvirus contain target gene fragment. SDS\|PAGE and Western blot analysis of the supernatant of the infected SF\|9 cell showed that KDR\-\{n3\} was secreted in the medium. The recombinant protein was verified with Western blot and tested for their binding activity with VEGF. Its anti\|angiogenic activity was assayed on chorionic allantoic membrane(CAM) of fertilized egg. The results showed that the recombinant protein could inhibit new vessel formation on CAM of fertilized eggs.
%K VEGF receptor
%K kDR
%K baculovirus<br>血管内皮细胞生长因子受体，
%K 克隆，
%K 杆状病毒表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=66E5AB96FE7B6D9C&yid=14E7EF987E4155E6&vid=BCA2697F357F2001&iid=0B39A22176CE99FB&sid=B0EBA60720995721&eid=3986B25773CB6C30&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=3&reference_num=16