%0 Journal Article
%T Site-directed Mutagenesis and Effects on the Enzymatic Properties of Subtilisin E<br>枯草蛋白酶E的定点突变及其对酶性质的影响
%A YANG Yong-hua
%A JIANG Lan
%A YANG Sheng-Li
%A WU Yu-Jie
%A ZHU Liu-Qin
%A <br>杨永华
%A 蒋岚
%A 杨胜利
%A 吴宇杰
%A 朱榴琴
%J 生物工程学报
%D 2000
%I 
%X Site\|directed mutagenesis was used to investigate the effects of S221C/P225A,N118S/S221C/P225A,D60N/N118S/S221C/P225A and Q103R/N118S/S221C/P225A mutations on the properties of Subtilisin E.It was found that S221C/P225A mutant is 73 000\|fold decreased in amidase activity than subtilisin E and 3\|fold increased than subtiligase in the ratio of esterase/amidase;N118S/S221C/P225A mutant has 3\^6\|fold and 15\|fold decreased in amidase and esterase activity respectively and as a result,it has a 4\|fold lower in the ratio of amidase/esterase than S221C/P225A mutant;Although it has no effect on the esterase activity,D60N/N118S/S221C/P225A mutant enhanced its ratio of amidase/esterase by 15 fold,3\^3\|fold and 10\^3 fold compared to N118S/S221C/P225A mutant,S221C/P225A mutant and subtiligase respectively;Q103R/N118S/S221C/P225A mutant,however,has a 5\|fold enhanced in the amidase activity and 55\|fold and 1000\|fold decrease in the esterase activity and the ratio of esterase/amidase compared to N118S/S221C/P225A.
%K Subtilisin E
%K site\|directed mutagenesis
%K amidase activity
%K esterase activity<br>枯草蛋白酶E，定点突变，蛋白酶活性，酯酶活性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=A70493C9346153FA&yid=9806D0D4EAA9BED3&vid=7801E6FC5AE9020C&iid=38B194292C032A66&sid=4B168891B5E5FB30&eid=A04F01817ECB9A48&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=15