%0 Journal Article
%T Extraction of the Salt-tolerant cDNA in Mangrove Avicennia marina by mRNA Differential Display<br>利用mRNA差别显示技术分离盐胁迫下红树植物白骨壤耐盐相关cDNA
%A ZHOU Han-Tao
%A LIN Peng
%A <br>周涵韬
%A 林鹏
%J 生物工程学报
%D 2002
%I 
%X RNAs of the leaves in Avicennia marina, which cultured in 50@1000 and 0@1000 salinity condition respectively, were isolated for mRNA differential display analysis. Screened by OligodT12 GC and eight 10-oligonucleotide arbitrary primers, differential cDNA fragments-csrg1(600 bp), csrg2(550 bp), csrg3(480 bp), only appeared in the leaves of Avicennia marina cultured in 50@1000 salinity condition. After detected by RNA dot hybridization, only csrg1 appeared the difference between the RNAs of the leaves in Avicennia marina cultured in 50@1000 and 0@1000 salinity condition respectively, and csrg1 was confirmed as the salt-tolerant cDNA. csrg1 was cloned and sequenced. After searching in Genbank, there were no similar sequences reported.
%K Avicennia marina
%K \% mRNA differential display
%K salt tolerance<br>白骨壤
%K mRNA差别显示
%K 耐盐性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=7EAD19BA50009304&yid=C3ACC247184A22C1&vid=13553B2D12F347E8&iid=CA4FD0336C81A37A&sid=987EDA49D8A7A635&eid=318E4CC20AED4940&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=5&reference_num=4