%0 Journal Article
%T Multiplication of the Shrimp Baculovirus HHNBV with Primary Cell Cultures from Lymphoid Organ of Penaeus chinensis<br>利用对虾原代细胞增殖对虾杆状病毒HHNBV的研究
%A MIAO Hong
%A Zhi TONG Shang
%A Liang
%A <br>苗宏志
%A 童裳亮
%A 徐斌
%A 姜明
%A 刘晓云
%J 生物工程学报
%D 2000
%I 
%X The hypodermal and hematopoietic necrosis baculovirus HHNBV has been confirmed to be the causative agent for the explosive epidermic disease of farmed shrimp Penaeus chinensis since 1993. The virus was isolated and multiplied successfully in the primary cell cultures from the lymphoid organ of the shrimp. A cell monolayer was formed in three days in the medium MPS and could be maintained for 1-3 months as the medium replaced every 4-5 days. The cytopathic effect occurred in 5 days after inoculation of the tissue extract from the diseased shrimp. The transmission electron microscopy showed many rod-shaped virions of HHNBV in the nuclei of infected cells.
%K Shrimp
%K cell culture
%K baculovirus
%K HHNBV<br>对虾
%K 细胞培养
%K 杆状病毒
%K HHNBV
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=E20E8A942585E2BE&yid=9806D0D4EAA9BED3&vid=7801E6FC5AE9020C&iid=0B39A22176CE99FB&sid=78F0EFE028BD3783&eid=A1266CF37D675CF1&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=4&reference_num=6