%0 Journal Article
%T Effect of Dissolved Oxygen on the Suspension Culture of Taxus chinensis<br>溶氧水平对红豆杉细胞悬浮培养的影响研究
%A LUO Jie
%A YU Fei
%A LIU Ling
%A WU Cheng
%A |De
%A MEI Xing
%A |Guo
%A <br>罗杰
%A 余斐
%A 刘凌
%A 吴诚德
%A 梅兴国
%J 生物工程学报
%D 2001
%I 
%X The effect of dissolved oxygen controlled level on the taxol production by suspended cell culture of Taxus chinensis was examined in shake flasks and in a 20L aerated and agitated bioreactor with a six-flat-bladed turbine. It was found in shake flasks that oxygen supply exhibited significant influence on the production of taxol. Further investigation in bioreactor showed that noly 1.5 mg/L taxol was produced when DO(dissolved oxygen) level was 20%. Taxol production increased when DO level was increased to 40%-60%. Moreover, taxol accumulation was remarkably improved by the cultivation of cells initially at the DO level of 60% during the growth phase for 14 days followed by changing the DO level to 20%-40% during the taxol production phase for another 10 days. An optimum taxol level of 7.2 mg/L was obtained by the dissolved oxygen controlled process.
%K Taxus chinensis
%K taxol
%K dissolved oxygen level control<br>红豆杉，
%K 紫杉醇，
%K 溶氧控制
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=D987B9A14B0DCB52&yid=14E7EF987E4155E6&vid=BCA2697F357F2001&iid=0B39A22176CE99FB&sid=1B64850025D0BBBE&eid=F9F74EC1AA08A7B9&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=4&reference_num=10