%0 Journal Article
%T The ex vivo Expansion of Cord Blood Hematopoietic Cells: 1. Cell Growth Behavior<br>脐血造血细胞的体外扩增：1.生长规律的研究
%A ZHANG Yuan-Xing
%A YING Xiao-Fei
%A TAN Wen-Song
%A <br>张元兴
%A 应小飞
%A 谭文松
%J 生物工程学报
%D 2000
%I 
%X The population growth and metabolism of hematopoietic cells from cord blood were investigated without and with hematopoietic growth factors and the long-term growth kinetics of cells was studied. In static cultivation of hematopoietic cells, the population specific growth rate was 0.34 d, and the average double time 2 days. The greater part of glucose was consumed and 40 mmol/L lactate was formed during cell culture. For the long-term culture, the maximum output of CFU-GM appeared between the 2nd and 3rd weeks and that of BFU-E in the first week. With 50% medium exchange, the expansions of total cells, CFU-GM, and BFU-E came up to 14, 13, and 5 times, respectively.
%K Hematopoietic cell
%K growth behavior
%K cell culture<br>造血细胞，生长动力学，细胞培养
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=C115780900358F16&yid=9806D0D4EAA9BED3&vid=7801E6FC5AE9020C&iid=94C357A881DFC066&sid=F8AEC975DBDD7F2F&eid=C824C8F9F54AE9B9&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=6