%0 Journal Article
%T High density fed-batch culture of Escherichia coli DH5 alpha/pDH-B2m with DO feed-back control of nutrient feeding]<br>溶氧反馈分批补料高密度培养人骨形成蛋白-2工程菌
%A LI Yi
%A PU Qin
%A ZHAO Zhong-Liang
%A CHAI Yu-Bo
%A CHEN Nan-Chun
%A CHEN Su-Min
%A <br>李毅
%A 蒲勤
%A 赵忠良
%A 柴玉波
%A 陈南春
%A 陈苏民
%J 生物工程学报
%D 2002
%I 
%X Optimization of cultivation condition of recombinant E. coli DH5 alpha/pDH-B2m and the condition suitable for expression of recombinant mature peptide of human bone morphogenetic protein-2 was carried out in 500 mL shaking flasks and then transferred to NBS Bioflo IV, a 20 L DO feed-back fed-batch culture system, to obtain rhBMP-2. The results indicate that keeping dissolved oxygen at 40% and controlling nutrient feeding rate with DO feed back strategy can obtain theoretically 3.59 g recombinant mature peptide of hBMP-2 per liter of broth, the final cell density OD600 reaches 57(22.8 g dry cell weight/L), and the expression of rhBMP-2 amounts to 30% of the total protein in E. coli.
%K human bone morphogenetic protein-2
%K recombinant E
%K coli
%K fed-batch
%K high density cultivation<br>溶氧反馈－分批补料
%K 高密度培养
%K 人骨形成蛋白－2
%K 工程菌
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=276E9D02A6FDD090&yid=C3ACC247184A22C1&vid=13553B2D12F347E8&iid=B31275AF3241DB2D&sid=5A027C8E6C570AAB&eid=19402779123D7C0E&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=2&reference_num=14