%0 Journal Article
%T Stable Expression of Erythropoietin cDNA in Chinese Hamster Ovary Cells<br>红细胞生成素在中国仓鼠卵巢细胞中稳定表达
%A Lu Baisong Xu Xiuying Chen Lin Huang Peitang
%A <br>卢柏松
%A 徐秀英
%A 陈琳
%A 黄培堂
%J 生物工程学报
%D 1996
%I 
%X An plasmid for the expression of erythropoietin is costructed by insering erthropoietin cDNA in cloning site of eucaryotic expression vector. The expression plasmid is introduced into COS-7 cells and biologically active erythropoietin is expressed, which is indicated by ELISA and colony formation assays. The plasmid is then introduced into CHO cells by means of electroporation and the foreign gene is amplified by exposing the cells to gradually higher concentration of methetrexate. The expression level reaches 2-3ug/106 cells/24hrs when MTX concentration is 2x10-7 mol/L. The expression level of the cells remain stable after 3 months of cryopreservation. It is possible to obtain higher expression level by further amplification and selection.
%K Erythropoietin cDNA
%K CHO cell
%K electroporation
%K stable expression<br>红细胞生成素
%K CHO细胞
%K 制备
%K 生物工程
%K 生物制品
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=EF90B7E4FF70CC97406542A07C11C81C&yid=8A15F8B0AA0E5323&vid=59906B3B2830C2C5&iid=38B194292C032A66&sid=717CC18E05F2AFA0&eid=9DC563A0FEFC04F9&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=4&reference_num=2