%0 Journal Article
%T STUDIES ON THE GEl. TECHNIQUE FOR GROWING OF LYSOZYME CRYSTALS<br>凝胶法生长溶菌酶晶体的研究
%A Han Qing Shen Fulin Gui Lulu Bi Ruchang
%A <br>韩青
%A 沈福苓
%A 桂璐璐
%A 毕汝昌
%J 生物物理学报
%D 1993
%I 
%X The gel medium could eliminate or reduce the convection and sedimentation caused by the gravity and it is worthwhile to explore the gel method used for crystal growth of biological macromolecules. Using hanging drop diffustion method, we have grown single large or perfect and highly symmetrical crystals of hen - egg white lysozyme. In the gel medium. MPD in low concentration could inhibit the nucleation in lysozyme crystallization, which is similar to that in solution procedure. The X - ray precession photography showed that the crystals grown in gel could diffract to a higher resolution.
%K Crystal growth Protein Gel method Lysozyme 2 - methyl - 2
%K 4 -pentanediol<br>晶体
%K 生长
%K 蛋白
%K 溶菌酶
%K 凝胶法
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=AA6910051ECACFFB38CF510F1F531DE2&yid=D418FDC97F7C2EBA&vid=9CF7A0430CBB2DFD&iid=E158A972A605785F&sid=F204392B3B11C3BD&eid=89389F643CA3F778&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=0&reference_num=3