%0 Journal Article
%T FLUORESCENCE STUDIES ON INTERACTION OF BPP WITH DMPC VESICLES<br>牛胰多肽(Bovine Pancreatic Polypeptide)和DMPC脂质体相互作用的荧光研究
%A 聂松青
%A 薄惠卿
%A 曾科
%A 林克椿
%A 顾增发
%J 生物物理学报
%D 1987
%I 
%X The present paper reports the interaction of bovine pancreatic polypeptide (BPP)with phospholipids by fluorescence methods.The results show:(1)fluor-escence spectra of DMPC Vesicles labeled with DNS-PE, 2-AS,6-AS, 12-AS reveal red shift and blue shift respectively,fluorescence intensity increases. (2) BPP prefers binding with zwitherionic DMPC to negatively charged PA.The disassociation constants are 2.8×10-7M and 5.0×10-7M respectively.(3) BPP decreased the P values of DPH-labeled DMPC below its phase transition tem-perature(Tm).The obvious Tm of DMPC is not observed with the binding of BPP to it.It seems that the binding of BPP to phospholipids are caused not only by electrostatic lipid-peptide interactionbut also hydrophobic interaction.This binding perhaps is an important factor by which BPP shows protection to pancreas cells.
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=6A4402013AC6F0EA82E409CE31879E19&yid=9C2DB0A0D5ABE6F8&vid=38B194292C032A66&iid=E158A972A605785F&sid=8C27CCA578E52082&eid=869B6F3117981EC4&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=0&reference_num=0