%0 Journal Article
%T Effects of Metal Ions on<br>金属离子对地衣芽孢杆菌合成多聚γ-谷氨酸的影响
%A G Yang
%A J Chen
%A Y B Qu
%A S Y Lun
%A <br>杨革
%A 陈坚
%A 曲音波
%A 伦世仪
%J 生物工程学报
%D 2001
%I 
%X In the course of gamma-poly (glutamic acid) gamma-PGA fermentation, metal ions K+, Mg2+, Fe3+, Ca2+, Mo6+, Mn2+, Co2+ and Zn2+ in the medium have certain effects on the synthesis of gamma-poly(glutamic acid). Excess or lack of K+, Mg2+ and Fe3+ results in reduced yield of gamma-PGA. It was found that the gamma-PGA synthesis by Bacillus licheniformis was promoted obviously by Ca2+ and Mo6+. Synthesis and stereochemical composition of gamma-PGA was greatly regulated by Mn2+. gamma-PGA was not produced without Mn2+ in medium, and with the increase of Zn2+ concentration the yield of gamma-PGA and the proportion of D-glutamic acid in the peptide increase. Regulative effect of Co2+ and Zn2+ was almost the same as that of Mn2+, thus the combination of Mn2+, Co2+ cannot enhance gamma-PGA synthesis and affect stereochemical composition of gamma-PGA. Based on the experimental date, an appropriate formulation of metal ions in the medium for gamma-PGA production was obtained.
%K 金属离子
%K 地衣芽孢杆菌
%K 多聚γ-谷氨酸
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=EFD77ACD76E5635C&yid=14E7EF987E4155E6&vid=BCA2697F357F2001&iid=B31275AF3241DB2D&sid=C19D5524C51D7FE4&eid=6452E1221020E61F&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=6&reference_num=3