%0 Journal Article
%T Effects of Glucose on Growth, Metabolism and EPO Expression in Recombinant CHO Cell Cultures<br>葡萄糖对重组CHO细胞生长代谢及EPO表达的影响
%A X M Sun
%A Y X Zhang
%A <br>孙祥明
%A 张元兴
%J 生物工程学报
%D 2001
%I 
%X The maximum viable cell density almost kept constant in the experimental range of initial glucose concentrations from 8.9 to 49.6mmol/L in the batch cultures of recombinant CHO cells. The yield coefficient of lactate to glucose increased with the initial glucose concentrations from 8.9 up to 17.9mmol/L, and then kept constant with above 17.9mmol/L. There was little effect of glucose on glutamine metabolism. It was also demonstrated that the accumulative concentration of EPO increased with the increase of glucose concentration from 8.9 to 17.9mmol/L, and decreased with its further increase from 17.9 to 49.6mmol/L. It is showed that there is an optimum glucose concentration at which the EPO express of cultured reombinant CHO cells is on the peak.
%K CHO cell
%K glucose
%K metabolism
%K erythropiotein
%K animal cell culture<br>CHO细胞
%K 葡萄糖
%K 代谢
%K 红细胞生成素
%K 动物细胞培养
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=027A9736543984BB&yid=14E7EF987E4155E6&vid=BCA2697F357F2001&iid=B31275AF3241DB2D&sid=6CDD207A90CE1EEC&eid=6C3EA4F7B6E5F836&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=3&reference_num=10