%0 Journal Article
%T Molecular Cloning and Expression of the cDNA Encoding Angiogenesis Inhibitor K4K5 with Pichia pastoris<br>血管生成抑制因子K4K5 Cdna基因的克隆及其在毕赤酵母中的表达
%A GUAN Xiao
%A |Qun
%A WANG Yue
%A |Xiang
%A WU Liang
%A |Cheng
%A SONG Hou
%A |Yan
%A <br>官孝群
%A 王跃祥
%A 吴良成
%A 宋后燕
%J 生物工程学报
%D 2001
%I 
%X Kringles of human plasminogen except kringle 4 can inhibit the endothelial cell growth. To determine whether recombinant plasminogen kringle 4-5 (r-K4K5) can inhibit the growth of bovine capillary endothelial (BCE) cell and the angiogenesis of chick embryo chorioallantoic membrane (CAM), we cloned and constructed the modified cDNA region encoding kringles 4 and 5 (K4K5) of human plasmin (ogen) and expressed it with pichia multi-copy expression system. One clone, with the most productive expression was selected from hundreds of transformants. Our data showed that the expression product r-K4K5 (MW 21.5 kD) amounted to 150-250 mg/liter, over 80% of the total secreted protein. It could, dose-dependently, inhibit BCE cell proliferation and inhibit chick embryo CAM angiogenesis.
%K K4K5 cDNA
%K cloning and expression
%K \%Pichia pastoris
%K \% angiogenesis<br>K4K5cDNA
%K 克隆表达
%K 毕赤酵母
%K 新生血管生成
%K 血管生成抑制因子
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=FAD08CD18EEFFCB0&yid=14E7EF987E4155E6&vid=BCA2697F357F2001&iid=0B39A22176CE99FB&sid=6FBD78E3BAB60869&eid=B47A0E731AF43EB2&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=12&reference_num=10