%0 Journal Article
%T cDNA Cloning,Fusion Expression in Escherichia coli and Activity Assay of hIL-11<br>人白细胞介素11 cDNA的克隆、融合表达及活性测定
%A ZHANG Ying
%A LIU Can-hui
%A LIU Yu-Le
%A TIEN Po
%A <br>张颖
%A 刘灿辉
%A 刘玉乐
%A 田波
%J 生物工程学报
%D 2000
%I 
%X Human Interleukin-11 (hIL-11) is a multifunctional cytokine which plays an important role in regulating the proliferation and differentiation of cells in the hematopoeitic, lymphoid system etc. To obtain the IL-11 cDNA, a primary culture of Chinese fetal lung fibroblast was prepared from fresh tissue. Then the human IL-11 cDNA without the N-terminal signal peptide sequence was cloned by RT-PCR from the cells induced by PMA. The sequence indicated that there are three bases different from those previously reported, but with no change of the amino acids. The cDNA was inserted into the 3' end of trxA gene in thioredoxin gene fusion expression system pTRXFUS to construct the trxA and hIL-11 fusion expression vector, and expressed in E. coli. The fusion hIL-11 accounts for more than 20% of the total bacteria proteins. The expression product is present in soluble forms and has the full biological and immunological activities.
%K Human interleukin 11 (hIL\|11)
%K primary culture of Chineses fetal lung fibroblast
%K RT\|PCR
%K thioredoxin gene fusion expression system
%K activity assay<br>人白细胞介素11
%K 活性测定
%K 基因克隆
%K 融合表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=D25089397B4206AD&yid=9806D0D4EAA9BED3&vid=7801E6FC5AE9020C&iid=38B194292C032A66&sid=A5111BA190517959&eid=35E8A259891FB32F&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=8