%0 Journal Article
%T AFLP Analysis of Photoperiod sensitive Genic Male Sterile(PGMS) Rice Mutant Lines<br>光敏核不育水稻等位突变系的AFLP分析
%A LI Chuan you ZHENG Hong gang
%A WENG Man li JIA Jian hang
%A MOU Tong min Henry TNguyen WANG Bin
%A <br>李传友
%A 郑洪刚
%A 翁曼丽
%A 贾建航
%A 牟同敏
%A y
%A T.Nguyen
%A 王斌
%J 生物工程学报
%D 2000
%I 
%X The reaction conditions for rice AFLP assay were optimized.The relative efficiencies for polymorphism detection of RFLP,RAPD and AFLP were compared through the analysis between a pair of PGMS allelic mutant lines(NK58S and NK58F).Results indicated that the efficiency for polymorphism detection in rice is in the order of AFLP>RAPD>RFLP,and also indicated that AFLP is a powerful DNA molecular marker technique for polymorphism detection,especially in the cases of extremely low polymorphism,such as isogeneic lines and allelic mutant lines.The advantages and disadvantages of these three molecular marker systems were discussed.Using AFLP in conjunction with bulked segregating analysis,5106 AFLP loci were screened and 9 of them showed polymorphism between NK58S and NK58F,4 of the polymorphic AFLP products were cloned,Southern bloting analysis showed that two of them were single copy sequences while the other two were low copy sequences in rice genome.
%K AFLP
%K rice
%K molecular marker
%K polymorphism detection efficiency<br>AFLP，水稻，分子标记，多态性检测效率
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=0599AEE00B40F94CE11940A9E414EC53&yid=9806D0D4EAA9BED3&vid=7801E6FC5AE9020C&iid=CA4FD0336C81A37A&sid=C753EB8AC8F551B9&eid=C36EC077A8A90308&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=5&reference_num=9