%0 Journal Article
%T Nutrition Condition of Hyaluronic Acid Fermentation with Streptococcus zooepidemicus<br>营养条件对兽疫链球菌发酵生产透明质酸的影响
%A GAO Hai-jun
%A CHEN Jian
%A ZHANG Yan-fang
%A DU Guo-cheng
%A <br>高海军
%A 陈坚
%A 章燕芳
%A 堵国成
%J 生物工程学报
%D 2000
%I 
%X Based on the analysis of metabolic pathway Streptococcous zooepidemicus for hyaluronic acid (HA) synthesis, nucleotide, especially uracil, was considered to be important to cell growth and metabolism. When 0.005 g.L-1 uracil added in the media in which yeast extract as complex nitrogen source, cell growth and HA production were increased by 32% and 34% respectively. From analysis of amino acid in fermentation process, it was show that arginine(Arg) was needed for cell metabolism, and concentration of free Arg maintained at 0 g.L-1 in fermentation process, which was proposed to limit cell growth and HA production. By shake-flask experiment HA concentration reached 0.510 g.L-1 when 0.06 g.L-1 Arg added, in the fermentation with 2.5 L fermentor, when uracil 0.005 g.L-1 and Arg 0.06 g.L-1 were added, the rate of cell growth increased, maximum of specific growth rate, concentration of HA and HA molecular weight reached 0.67 h-1, 5.2 g.L-1 and 2.15 x 10(6) Da from 0.54 h-1, 4.2 g.L-1, 2.0 x 10(6) Da, respectively.
%K Streptococcus zooepidemicus
%K hyaluronic acid
%K fermentation
%K nutrition<br>Streptococcus
%K 
%K 透明质酸，环境，影响
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=38D8A958060C94AE&yid=9806D0D4EAA9BED3&vid=7801E6FC5AE9020C&iid=38B194292C032A66&sid=7D6CD8918B045FD4&eid=87545994EC2C1F12&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=15&reference_num=13