%0 Journal Article
%T Research on Renaturation of Recombinant Human Pro-urokinase Expressed from Escherichia coli<br>重组人尿激酶原的体外变复性研究
%A ZHU Hui
%A LIU Wei
%A SHI Wei
%A XUE Yu
%A Ming
%A MA Zhong
%A <br>朱慧
%A 刘伟
%A 史蔚
%A 薛宇鸣
%A 马忠
%J 生物工程学报
%D 2000
%I 
%X Recombinant human pro-urokinase forms insoluble inclusion body when overexpressed in Escherichia coli, and it must be denatured and renatured in vitro before it acquires activity. This study aimed to increase the renaturation yield of denatured pro-urokinase. We have evaluated the basic renaturation conditions of pro-urokinase through qualitative and quantitative analysis of pH, temperature, denaturant concentration, protein concentration, the ratio of reduced and oxidized thiol reagents. The effects of nonspecific additives, step-wise dilution and urea gradient dialysis have been also compared. The optimal conditions of pro-urokinase renaturation with the yield about 20%-30% have been obtained.
%K Recombinant human pro
%K urokinase
%K denaturation
%K renaturation<br>重组人尿激酶原
%K 变性
%K 复性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=EA0FB105655A1334&yid=9806D0D4EAA9BED3&vid=7801E6FC5AE9020C&iid=0B39A22176CE99FB&sid=2DBBF45CC176713E&eid=E2546871E5B846EF&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=4&reference_num=12