%0 Journal Article
%T Agrobacterium-mediated transformation of Orychophragmus Violaceus Cotyledon and Regeneration of Transgenic Plants<br>根癌土壤杆菌介导转化诸葛菜子叶获得转基因植株
%A Zhou Jiming Wei Zhimfng Xu Zhihong Liu Shigui Luo Peng
%A <br>周冀明
%A 卫志明
%A 许智宏
%A 刘世贵
%A 罗鹏
%J 生物工程学报
%D 1996
%I 
%X Excised cotyledons of Orychophragmus violaceus, were used as explants for tissue culture. They were cultured on MS medium supplemented with BA 3 mg/L and NAA 0. 2mg/L. When the regenerated buds were 2cm long, they were excised and transferred onto 1/2MS medium with IBA 0. 03mg/L, then the whole planticts were regenerated . The frequency of plant regenration was 100%. Then we began to study the genetic transformation of O. violaceus, After 2-3 days of cocultivation with Agrobacteri-um tumefaciens strain A208se (pTiT37 pROA93), the cotyledons were transferred onto the selection medium containing 25mg/L Km and 250mg/L Ap. After shoots emerged, they were excised and transferred onto the root medium containing 25mg/L Km and 100mg/L Cef, the roots were formed within 4-5 weeks. The whole plants were transplanted into pots and grew well. The frequency of plant regeneration was about 51%. The regenerated plants showed high enzymatic activities of B-glucuronidase and neomycine phosphotransferase I . Southern blot analysis confirmed that NPT I gene had been stably integrated into the chomosomal genome of O. violaceus. The transformation frequency was 5. 6%. In this paper, the first transgenic plant of O. violaceus is being reported.
%K Agrobacteriurn tumefaciens
%K Orychopheagmus violaceus
%K cotyledon
%K trans-genie plants<br>根癌土壤杆菌，诸葛菜，子叶，转基因植株
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=EF90B7E4FF70CC97BEEFB7F146293D64&yid=8A15F8B0AA0E5323&vid=59906B3B2830C2C5&iid=CA4FD0336C81A37A&sid=339D79302DF62549&eid=7C3A4C1EE6A45749&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=12&reference_num=6