%0 Journal Article
%T A Construct for the Trial Expression of Streptomyces PKS Gene in Plant<br>一个可介导链霉菌PKS基因 向植物转化的杂合质粒的构建
%A Tao Meifeng Hu Zhihao Zhou Xiufen Deng Zixin David A Hopwood Tobias Kieser
%A <br>陶美凤
%A 胡志浩
%A 周秀芬
%A 邓子新
%J 生物工程学报
%D 1996
%I 
%X 3. 8kb DNA immediately downstream of pabAB genes was sequenced and it was confirmed that the FR-008 antibiotic was synthesiecl by Type 1 PKS. We were interested to see whether the FR-008 PKS genes can be expressed in plant. The sequence information was thus used to make a construct for gene expression in plant. Plasmid WRG2410 containing 35S promoter was chosen as a vector to construct pHZ321 which is now ready for introdction into plant. At this stage, we neither expect the introduced construct in plant to produce a functional enzyme, nor tc confer fungal resistance to plant, but it will give some indication whether the Streptomyces PKS gene, which has an extremely high G C content (76%) can be expressed in various plants.
%K PKS
%K Streptomyces<br>农业生物工程
%K 链霉菌
%K 植物转化
%K PKS基因
%K 质粒
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=EF90B7E4FF70CC971A06FD2246DA0B78&yid=8A15F8B0AA0E5323&vid=59906B3B2830C2C5&iid=E158A972A605785F&sid=4A2356A1257A12EB&eid=3F419E61BD389CC8&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=2