%0 Journal Article
%T Cloning of a Gene Encoding the Precursor of Nisin by PCR<br>利用PCR技术克隆乳链菌肽前体基因
%A Huan Liandong Tao Yong Tian Yuqing He Song Xue Yugu
%A <br>还连栋
%A 陶勇
%A 田宇清
%A 何松
%A 薛禹谷
%J 生物工程学报
%D 1995
%I 
%X The structural gene for the precursor of nisin was synthesized by polymerase chain reaction and then cloned in pUC18. The nucleotide sequence of the cloned precursor nisin gene was determined by dideoxy termination method. The sequence data obtained agreed with those of precursor nisin genes isolated by other workers from different Lactococcus lactis strains.
%K Lactococcus lactis
%K nisin precursor gene
%K PCR<br>乳链菌肽
%K 前体基因
%K 克隆
%K 聚合酶链反应
%K 基因工程
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=94568B1E88D2D812EF73D58C6FC8BF42&yid=BBCD5003575B2B5F&vid=708DD6B15D2464E8&iid=E158A972A605785F&sid=40862C82D74F7473&eid=AFB2C35009D5D191&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=3&reference_num=0