%0 Journal Article
%T Study on Alkaline Protease Production by Immobilized Serratia marcescens in Carrageenan<br>卡拉胶固定粘质赛氏菌产碱性蛋白酶的研究
%A Feng Li Chen Xiangdong Peng Zhenrong
%A <br>冯立
%A 陈向东
%A 彭珍荣
%J 生物工程学报
%D 1994
%I 
%X The strain Serratia marcescens S3 was immobilized in carrageenan to produce alkaline protease and the optimum concentration of carrageenan was 2. 5%. Under the optimum culture conditions, the protease activity reached about 400u/ml. By adding 1% soybean meal and 3% corn meal or 2% sands to carrageenan, 25% and 23. 9% higher protease activities could be realized. The half life of producing proteasee by immobilized cells was 14 times (24h percycle) in culture flask simicoritinuous fermentation, and 52 times (12h per cycle) in liquid cycling apparatus fermentation, The efficiencies of producing protease in these two processes were 11. 8% and 45. 07% higher than that by free cells in'culture flask batch fermentation. Moreover, the efficiency of producing protease in liquid cyclying appartus fermentation was 29. 7% higher than that in culture flask simicontinuous fermentation.
%K Immobilization
%K alkaline protease
%K Serratia marcescens
%K carrageenan<br>固定化细胞，卡拉胶，粘质赛氏菌，碱性蛋白酶
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=4DA8D0874F036D8116A8969E1F2A41DF&yid=3EBE383EEA0A6494&vid=F3090AE9B60B7ED1&iid=CA4FD0336C81A37A&sid=5C3443B19473A746&eid=09ABD5535D9B6D45&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=4&reference_num=1