%0 Journal Article
%T Studies on High Salt Tolerance of Transgenic Tobacco<br>高度耐盐双价转基因烟草的研究
%A Liu Junjun Huang Shaoxing Peng Xuexian Liu Weibo Wang Haiyun
%A <br>刘俊君
%A 黄绍兴
%A 彭学贤
%A 柳维波
%A 王海云
%J 生物工程学报
%D 1995
%I 
%X With E. coll mtlD gene (encoding mannitol-1-phosphate dehydrogenase) and gutD gene (encoding glucitol-6-phosphate dehydrogenase)cloned ,plant expression vector pBIGM had been obtained by inserted mtlD and gutD genes into binary vector pBin438. Tobacco was transformed with A. tumefaciens LBA4404 containing pBIGM. Results of molecular hybridization of transformed plants indicated that mtlD and gutD genes had integrated into the genomic DNA of tobacco plants. Experiments of salt tolerance and analysis of sugar alcohols showed that accumulation of different sugar alcohols in trans-genie tobacco plants had increased salt tolerance of tobacco.
%K E
%K coli gutD gene
%K mtlD gene
%K transgenic tobacco
%K high salt tolerance<br>烟草
%K 转基因烟草
%K 耐盐性
%K 农业生物工程
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=6FE04395076FF8768A05CB4323C12493&yid=BBCD5003575B2B5F&vid=708DD6B15D2464E8&iid=E158A972A605785F&sid=B9B90065CF5CD7F0&eid=05340B75C67FF664&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=29&reference_num=5