%0 Journal Article
%T Expression of Goldfish Growth Hormone I Gene Using Baculovirus Expression System<br>利用杆状病毒表达系统表达金鱼生长激素I基因
%A Lin Guangyun
%A Wang Xunzhang
%A Anderson Wong
%A Long Qingxin
%A Pang Yi
%A Yu Keili
%A <br>林广云 王珣章 Anderson Wong 龙綮新
%A 庞义 余奇理
%J 生物工程学报
%D 1997
%I 
%X Using pSXIVVI +X3 without an initiation codon as an expressing vector, an occluded recombinant Trichoplusia ni nuclear polyhedrosis virus carrying the cDNA encoding goldfish growth hormone I (gf GHI) under the control of the Syn XIV promoter, has been constructed. Immunoblot analysis showed that the molecular weight of the expressed protein was 22 5kDa as expected, revealed that the virus mediated gfGHI was synthesized in the Sf cells, larvae and secreted into the supernatant and hemolymph.
%K Goldfish growth hormone
%K baculovirus
%K expression
%K secretion<br>金鱼生长激素．杆状病毒，表达，分泌
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=DAF48826267185B63167618BCA152AB4&yid=5370399DC954B911&vid=FC0714F8D2EB605D&iid=0B39A22176CE99FB&sid=EB552E4CFC85690B&eid=D59111839E7C8BDF&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=5&reference_num=2