%0 Journal Article
%T Protoplast Culture and Plant Regeneration from the Suspension Cells of Gynostemma pentaphyllum (Thumb) Mak.<br>绞股蓝悬浮细胞的原生质体再生植株
%A Zhang Hangning Wu Qinsheng Liu Dajun
%A <br>张航宁
%A 吴琴生
%A 刘大钧
%J 生物工程学报
%D 1995
%I 
%X While the embryogenic callus induced from young stems of G. pentaphyllum on MS medium with Img/L 2,4-D and 0. 2mg/L KT were cultured in MS liquid medium on a rotary shaker, stable embryogenic suspensions were establidshed. A high yield of protoplasts could be released from the suspension cells incubated in a enzyme solution containing 2%cellulase RS, 0. 1% pectolyase Y-23, 0. 55mol/L mannital and CPW salt for 5 hours. These protoplasts were cultured in defferent medium with liquid and nurse culture method. Cell division was observes within 4 days and microcalli were formed within 4 weeks. After proliferating, protoplast-derived callus differentiated into embry-oid on MS solid medium with Img/L KT and 0. 5mg/L IAA. Stems and leaves were formed on MS medium with Img/L 6-BA and 0. 5mg/L IAA. Finally, complet plantlets were obtained on hormon free MS solid medium.
%K Gynostemma pentaphyllurn (Thumb) Mak
%K suspension cells
%K protoplast
%K plant
%K regeneration<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=CBB2F3D081380FD043D64BF04E342EF2&yid=BBCD5003575B2B5F&vid=708DD6B15D2464E8&iid=38B194292C032A66&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=0