%0 Journal Article
%T The Factors Affected Transformation Efficiency of Coryneform Bacteria by Electroporation<br>棒状类细菌电击转化中多种条件对转化效率的影响
%A Shen Tianxiang Na Shumin Xiao Wenzhong Jia Panxing
%A <br>沈天翔
%A 那淑敏
%A 肖文中
%A 贾盘兴
%J 生物工程学报
%D 1995
%I 
%X High voltage electroporation was performed to transfer plasmid DNA of pXZl0145 into different kinds of corynebacteria strains. A number of factors that affected transformation efficiency were investigated. Cell grown in the presence of 4% glycine and harvested in the early exponential growth phase (OD600 was about 0. 2) was much easier to be transform. Transformation efficiency of up to 8X106 transformants per fig plasmid DNA with homologously derived DNA was obtained. If nonhorhologously derived DNA was used, transformation efficiency was 102 to 103 times lower than the former one.
%K Coryneform bacteria
%K electroporation
%K transformation efficiency<br>棒状类细菌，高压电击转化，转化效率
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=8B0FDF1C0E10D5A1C2D1B0824596B4A4&yid=BBCD5003575B2B5F&vid=708DD6B15D2464E8&iid=38B194292C032A66&sid=E2E0FBFE4D7EFB94&eid=D537C66B6404FE57&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=9&reference_num=2