%0 Journal Article
%T Fusion Expression Vectors for the Recombinant Gene Products Processed Easily and Purified Rapidly by the Affinity Chromatography<br>易于基因产物加工和快速纯化的融合表达载体
%A Li Boliang Yang Xinying Jiang Zhihong Hu Ming Liang Zhenhe
%A <br>李伯良
%A 杨新颖
%A 江智红
%A 胡明
%A 梁镇和
%J 生物工程学报
%D 1994
%I 
%X A DNA fragment encoding IgG-binding domain B, C (PABC) was separated from protein A gene, cloned into phage M13 and modified by oligo-directed mutagenesis at the hydroxylamine-cleaved site from Asn-Gly to Asn-Ala in domain B and C, respectively. The modified PABCm gene fragment was used to construct one set of fusion expression vectors in different reading frame. Some processing sequences such as those recognized by enterokinase, collagenase, thrombin, activated factor X and cleaved by the hydroxylamine, N-chlorosuccinimide etc. can be created in the fusion site. Using the above vectors, some fusion proteins such as PABCm-IGF-I, -hGRF, -bGRF and their derivatives were highly expressed in E. colt. The yield of fusion proteins is over 100mg per liter culture by analysis of SDS-PAGE. The PABC fusion proteins can be rapidly purified by the affinity chromatography with a IgG-sepharose 4B column.
%K Protein A
%K site-specific mutagenesis
%K fusion expression<br>Protein
%K A，点突变，融合表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=0795B99E992E80EEA1A46CB68D5574FB&yid=3EBE383EEA0A6494&vid=F3090AE9B60B7ED1&iid=38B194292C032A66&sid=9F6DA927E843CD50&eid=D2742EEE6F4DF8FE&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=3&reference_num=3