%0 Journal Article
%T Gene Cloning and Sequencing of the Variable Region of Human Monoclonal Antibody Against Hemorrhagic Fever Virus<br>抗HFRSV人单抗可变区基因克隆及其序列测定
%A Gao Lei Chen Sumin Chen Nanchun Yang Angang Cui Yunchang
%A <br>高磊
%A 陈苏民
%A 陈南春
%A 杨安钢
%A 崔运昌
%J 生物工程学报
%D 1994
%I 
%X Human-Human hybridoma cell line 87-2 secretes anti HFRSV human monoclonal antibody. Total RNA was extracted from the cell and was reverse transcribed to the first strand cDNA using oligo d (T) as primer. A set of oligqnucleotide primers were designed to amplify the cDNA of human immunoglubulin heavy and light chain variable domains by polymerase chain reaction. The amplified VH and VL fragments were ligat-ed to the phage M13 vector DNA. The clones containing V gene inserts were sequenced. The sequences were confirmed as the human immunoglubulin variable genes by comparing with those of known sequences . There are framework (FR) and complementarity determining (CDR) regions in the variable genes.
%K Human monoclonal antibody
%K variable region
%K gene sequence<br>肾综合症出血热病毒，人源性单克隆抗体，
%K 抗体可变区．基因序列
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=0795B99E992E80EE640B6AB32AC63951&yid=3EBE383EEA0A6494&vid=F3090AE9B60B7ED1&iid=E158A972A605785F&sid=302684F6FB4B24FF&eid=4B168891B5E5FB30&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=2