%0 Journal Article
%T Large-scale Culture of Arnebia euchroma (Royle) Johnston Cells in 5L Outloop Airlift Bioreactor<br>外循环气升式反应器培养新疆紫草细胞
%A Chen Shiyun Hou Songsheng Ye Hechun Li Guofeng Zhang Jian Li Xinming Chen Lu Dong Jiaowang
%A <br>陈士云
%A 侯嵩生
%A 叶和春
%A 李国凤
%A 张健
%A 李新明
%A 陈路
%A 董教望
%J 生物工程学报
%D 1994
%I 
%X A two-stage culture method was established to culture Arnebia euchroma (Royle) Johnston cells in suspension and 5L outloop airlift bioreactor. Relationship between cell growth, shikonin formation and changes of conductivity, soluble sugar in cultured medium were studied. At the first stage, cells grew very fast, with the formation of a small amount of shikonin derivatives, and conductivity and soluble sugar decreased sharply. After cultured the cells in the second stage, conductivity decreased at first, and then grew up after a large amount of shikonin derivatives formed and secreted into the medium. By minotoring the changes of conductivity and soluble sugar, cell growth and shikonin derivatives formation were estimated in situ during the large-scale culture of A. euchroma cells.
%K Arnebia euchroma (Royle) Johnston
%K suspension culture
%K bioreactor culture
%K conductivity
%K soluble sugar<br>新疆紫草，悬浮培养，反应器培养，电导率．可溶性糖
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=0795B99E992E80EE5E27A3E9212264FE&yid=3EBE383EEA0A6494&vid=F3090AE9B60B7ED1&iid=CA4FD0336C81A37A&sid=35FC3610259C2B32&eid=7AA74D31F1FF2DCE&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=9&reference_num=4