%0 Journal Article
%T CONSTRUCTION OF SUBSTRACTED cDNA LIBRARY OF LITOPENAEUS VANNAMEI INDUCED BY VIBRIO ALGINOLYTICUS AND ANALYSIS OF EXPRESSED SEQUENCE TAGS<br>溶藻弧菌诱导凡纳滨对虾消减文库的构建及其表达序列标签分析
%A CAI Xiao-Hui
%A LU Yi-Shan
%A WU Zao-He
%A JIAN Ji-Chang
%A WANG Bei
%A CAI Shuang-Hu
%A <br>蔡小辉
%A 鲁义善
%A 吴灶和
%A 简纪常
%A 王蓓
%A 蔡双虎
%J 水生生物学报
%D 2010
%I 
%X Vibrio alginolyticus is one of the main causative agents resulting in serious infectious diseases of Litopenaeus vannamei and other animals.Suppression subtractive hybridization(SSH) is a new and highly effective method for analysing differentially expressed or tissue-specific cDNA probes and libraries.It is applicable to diagnosis of disease,development,tissue specificity,or other differential expressing.Based on the user manual of Clontech PCR-SelectTM cDNA subtraction kit,a suppression subtraction cDNA l...
%K Litopenaeus vannamei
%K Vibrio alginolyticus
%K Suppression subtractive hybridization (SSH)
%K Expressed sequence tag (EST)<br>萍乡肉红鲫
%K 受精
%K 精子入卵
%K 胚胎发育
%K 凡纳滨对虾
%K 溶藻弧菌
%K 抑制消减杂交(SSH)
%K 表达序列标签(EST)
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=256651267D5D2E4520A7707D05E98747&aid=3246B33D208792BAA172886DD936FCAB&yid=140ECF96957D60B2&vid=339D79302DF62549&iid=CA4FD0336C81A37A&sid=0B4F496D54044D86&journal_id=1000-3207&journal_name=水生生物学报&referenced_num=1&reference_num=28