%0 Journal Article
%T Combined effect of temperature and salinity on the Na+-K+-ATPase activity from the gill of GIFT tilapia juveniles(Oreochromis niloticus)<br>温度和盐度对吉富品系尼罗罗非鱼幼鱼鳃Na+-K+-ATPase活力的联合效应
%A WANG Haizhen
%A WANG Hui
%A QIANG Jun
%A XU Pao
%A LI Ruiwei
%A <br>王海贞
%A 王辉
%A 强俊
%A 徐跑
%A 李瑞伟
%J 生态学报
%D 2012
%I 
%X Central composite face-centered design(CCF) and response surface methodology were used in the experiment to examine the combined effect of temperature(12—34℃) and salinity(0—26) on the Na+-K+-ATPase activity from the gill of tilapia juveniles(GIFT strain)(mean body length(4.3±0.105)cm and mean body weight(2.45±0.153)g).The results showed that:(1)The linear and quadratic effects of temperature and salinity on the Na+-K+-ATPase activity were highly significant(P<0.01),and the interactive effect between temperature and salinity was nonsignificant(P>0.05);(2)Using the response surface method,the Na+-K+-ATPase activity varied with increasing temperature and salinity in a curvilinear way;(3)The model equation of the enzyme activity towards temperature and salinity was established,with R2=0.9829 and Pred.R2=0.855(P<0.01).The model equation could be practically used for forecasting the gill Na+-K+-ATPase activity in GIFT Nile tilapia juveniles;(4)Via applying statistical optimization of the response of interest,results showed that the optimal factor combination was 24.15℃/11.75,at which the minimum Na+-K+-ATPase activity,0.62μmolPi·mg-1pr·h-1,was reached,with the desirability as high as 0.961.The Na+-K+-ATPase activity can be used as an indicator of growth of GIFT Nile tilapia juveniles.In general,It can be said that the environment in which the Na+-K+-ATPase activity is weaker is suitable for the tilapia juvenile growth,and in this case the metabolism of tilapia is more efficient,and the energy consumed for osmoregulation is smaller.
%K GIFT Nile tilapia (Oreochromis niloticus )
%K temperature
%K salinity
%K Na+-K+-ATPase
%K response surface method<br>吉富罗非鱼
%K 温度
%K 盐度
%K Na+-K+-ATPase
%K 响应曲面法
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=FE163E5DB2274E5937319DE98913EC37&aid=4A3EF6807D65F871B67054AFEFFF3892&yid=99E9153A83D4CB11&vid=9971A5E270697F23&iid=38B194292C032A66&sid=9124D83E61CF1CD0&eid=0FCE62EC9DC00F5E&journal_id=1000-0933&journal_name=生态学报&referenced_num=0&reference_num=44