%0 Journal Article %T Template-Directed Ligation of Tethered Mononucleotides by T4 DNA Ligase for Kinase Ribozyme Selection %A David G. Nickens %A Nirmala Bardiya %A James T. Patterson %A Donald H. Burke %J PLOS ONE %D 2012 %I Public Library of Science (PLoS) %R 10.1371/journal.pone.0012368 %X In vitro selection of kinase ribozymes for small molecule metabolites, such as free nucleosides, will require partition systems that discriminate active from inactive RNA species. While nucleic acid catalysis of phosphoryl transfer is well established for phosphorylation of 5กไ or 2กไ OH of oligonucleotide substrates, phosphorylation of diffusible small molecules has not been demonstrated. %U http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0012368