%0 Journal Article
%T Population genetic diversity of the insular plant Neolitsea sericea based on random amplified polymorphic DNA (RAPD)<br>岛屿植物舟山新木姜子居群遗传多样性的RAPD分析
%A WANG Zhong-Sheng
%A <br>王中生
%J 生态学报
%D 2004
%I 
%X The Zhoushan archipelago is the largest archipelago in China. It separated from the mainland about 9000 years ago due to rising sea level and climate change. Because of the long-term influences of human activities, forest vegetation on the larger islands was badly damaged and plant diversity reduced. The remaining plants are deserving of attention, especially the 21 endangered plant species distributed in the archipelago. Genetic diversity and genetic differentiation were examined in six populations of the insular endangered plant Neolitsea sericea in Zhoushan archipelago using random amplified polymorphic DNA (RAPD) markers. A total of 84 discernible loci were obtained for all populations using 10 primers, 38.10% of which were polymorphic (PPL=38.10%). As analyzed by POPOGENE, average percentage of polymorphic loci (PPL=23.18%), Nei's genetic diversity (H_E=0.0793) and Shannon's information index (H=0.1201) indicated that Neolitsea sericea had a lower level of genetic diversity than other insular plants. The high value of differentiation (Gst=0.3646) indicated that geographic isolation strongly influenced genetic differentiation among populations, and a significant correlation was found between genetic distance and geographic distance (r=0.7697,p=96.62%). The combined information from analyses of genetic diversity and UPGMA indicated that some individuals on Putu Island were immigrants from Damao Island, and that populations on Zhujiajian Island had been translocated from Putu Island by human activities. Management strategies were proposed for species conservation and resource utilization for Neolitsea sericea. These included (a) in situ protection from human disturbance to facilitate natural regeneration, and (b) construction of germplasm resources and cultivate plants collected from insular populations for use to enhance gene flow, and to select elite lines for vegetation restoration and gardening applications on islands.
%K island
%K Neolitsea sericea
%K RAPD
%K genetic diversity
%K population differentiation
%K geographical isolation<br>岛屿
%K 舟山新木姜子
%K RAPD
%K 遗传多样性
%K 居群分化
%K 地理隔离
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=FE163E5DB2274E5937319DE98913EC37&aid=476C0AEF37817BD9&yid=D0E58B75BFD8E51C&vid=B91E8C6D6FE990DB&iid=38B194292C032A66&sid=3081401A9FAB9CE2&eid=B941678158018439&journal_id=1000-0933&journal_name=生态学报&referenced_num=10&reference_num=46