%0 Journal Article
%T Genetic Differentiation in Leymus chinensis Populations Revealed by RAPD Markers Ⅱ. Statistics Analysis<br>羊草种群遗传分化的RAPD分析Ⅱ.RAPD数据的统计分析
%A CUI Ji-Zhe
%A <br>崔继哲
%J 生态学报
%D 2002
%I 
%X Leymus chinensis (Trin.)Tzvel., which is one of rhizome grass species, has two ecotypes in the Songnen Plain,including a gray green type and a yellow green type. The levels of genetic diversity and genetic differentiation based on RAPDs were assessed among seven populations of the gray green type and two populations of the yellow green type (a total of 105 individuals). Fifteen 10-mer primers screened from seventy Operon's primer series were used to amplify RAPD fragments using a standard RAPD protocol. The number of amplified fragments and the percentage of polymorphic loci were different among nine populations. Populations that belong to the yellow green type possessed fewer amplified fragments (<90) and lower percentage of polymorphic loci(<50%)than that of the gray green type (>100 and >70% respectively). Genetic diversity based on RAPD phenotypic or genotypic frequencies was calculated using seven published methods by the statistical package RAPDISTANCE. The genetic diversity level in the yellow green type was lower than that in the gray green type. F_ IS value previously estimated with codominant markers, e.g.allozymes for the same population was used to estimate the null-allele frequencies for putative RAPD loci corrected for potential deviations from Hardy-Weinberg equilibrium. Shannon's information index and Nei's index of genetic diffferentiation were used to partition diversity within and among population components, and 37.6% or 35.7% of the variation detected with RAPDs was partitioned among populations. Genetic identity between the populations of two types were both on 0.8, while the genetic indentity within the gray green populations was above 0.9. The results achieved by the methods of either allozymes previously adopted or RAPDs revealed the same genetic pattern of variation. These results at the DNA level were parallel to those at the protein level. At both the protein and DNA levels, genetic polymorphism was higher in the populations of the gray green type than that in the yellow green populations.
%K Leymus chinensis
%K RAPD
%K genetic diversity
%K genetic differentiation<br>羊草种群
%K 遗传分化
%K RAPD分析
%K 数据
%K 统计分析
%K 遗传多样性
%K 草原
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=FE163E5DB2274E5937319DE98913EC37&aid=7BB2D02D8C0FFFDC&yid=C3ACC247184A22C1&vid=BC12EA701C895178&iid=DF92D298D3FF1E6E&sid=7D89C25BE291E00C&eid=A73A882009D0AEFE&journal_id=1000-0933&journal_name=生态学报&referenced_num=9&reference_num=25