%0 Journal Article
%T Development in research methods of microbial ecology<br>微生物生态学研究方法进展
%A ZHANG Hong-Xun
%A <br>张洪勋
%J 生态学报
%D 2003
%I 
%X The cultivation of microbes and microscopy techniques as a means to identify microbial communities have severe limitations, since the majority of microbes in the environment are viable but nonculturable. For this reason, the methods of biochemistry and molecular biology that are independent of culturing are being widely used in the studies of microbial ecology. In this paper, fluorescent technique, PCR-based technique, PLFA technique and others to characterize diversity of microorganisms are reviewed. ...
%K microbial ecology
%K GFP
%K FISH
%K PCR-RFLP
%K PCR-SSCP
%K PCR-DGGE
%K PLFA<br>微生物生态学
%K 研究方法
%K 显微技术
%K 微生物培养
%K 生物化学
%K 分子生物学方法
%K PCR
%K PLFA
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=FE163E5DB2274E5937319DE98913EC37&aid=DE81D5F2A07B39DC&yid=D43C4A19B2EE3C0A&vid=EA389574707BDED3&iid=94C357A881DFC066&sid=BB44F42BE8AE7430&eid=342E193BAB6B28C5&journal_id=1000-0933&journal_name=生态学报&referenced_num=34&reference_num=54