%0 Journal Article
%T DNA Extraction from Activated Sludge for Molecular Community Analysis<br>可用于微生物群落分子生态学研究的活性污泥总DNA提取方法研究
%A GAO Ping-Ping
%A <br>高平平
%J 生态学报
%D 2002
%I 
%X A rapid and less instrument\|dependent protocol for direct extraction of DNA from activated sludge sample was developed. To optimize the cell lysis step, four cell lysis treatments (Freeze\|thaw, lysozyme, SDS , and thermal shock +lysozyme + SDS) were used to disrupt dispersed cells and release DNA. The efficiency of cell lysis was assessed microscopically after each treatment step. The quality and quantity of extracted DNA was determined by using both agarose gel electrophoresis and spectrophotometric meas...
%K activated sludge
%K DNA extraction
%K PCR amplification<br>微生物群落
%K 分子生态学研究
%K 活性污泥总DNA
%K 提取方法
%K 环境微生物
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=FE163E5DB2274E5937319DE98913EC37&aid=6038AAC6009E29AE&yid=C3ACC247184A22C1&vid=BC12EA701C895178&iid=708DD6B15D2464E8&sid=FFD10F7019FAA9EC&eid=B6351343F4791CA3&journal_id=1000-0933&journal_name=生态学报&referenced_num=45&reference_num=9