%0 Journal Article
%T A protein extraction method suitable for two_dimensional electrophoresis analysis of halophytes<br>适用于盐生植物的双向电泳样品制备方法
%A LI Xiao-Fang
%A HAN He-Ping
%A WANG Xu-Chu
%A FAN Peng-Xiang
%A LI Yin-Xin
%A <br>李肖芳
%A 韩和平
%A 王旭初
%A 范鹏祥
%A 李银心
%J 生态学报
%D 2006
%I 
%X We compared three different methods,i.e.trichloroacetic acid/acetone precipitation(TCA),trichloroacetic acid precipitation(E-TCA), and phenol extraction(Phe),to determine their efficiency in extracting total proteins from Salicornia europaea L.by two-dimensional electrophoresis(2-DE) analysis.Generally,579,343,and 535 protein spots were generated by the three methods.The 2-DE gels of TCA and E-TCA showed strong horizontal streaks,while that of the Phe method had only a few faint streaks,which indicated effective removal of salt from the protein sample by the Phe method.Moreover,an optimized extraction buffer of the Phe method was capable of generating more protein spots and gave more clear background in the 2-DE map.Findings from this study are of great significance for developing effective protein extraction methods for other halophytes,and halophilic microorganisms.
%K protein extraction
%K two-dimensional electrophoresis(2-DE)
%K Phenol
%K Trichloroacetic acid(TCA)
%K Salicornia europaea L<br>蛋白质提取
%K 双向电泳(2-DE)
%K 酚
%K 三氯乙酸(TCA)
%K 盐角草
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=FE163E5DB2274E5937319DE98913EC37&aid=81FA15ACBF356B3B&yid=37904DC365DD7266&vid=96C778EE049EE47D&iid=B31275AF3241DB2D&sid=47AB527E821B9862&eid=CE651EF760BCA96C&journal_id=1000-0933&journal_name=生态学报&referenced_num=8&reference_num=15