%0 Journal Article %T RAPD analysis of genetic diversity of Kobresia humilis along the eastern of QinghaiTibet Plateau of China
青藏高原东部矮生嵩草遗传多样性的RAPD研究 %A ZHAO Qing-Fang %A LI Qiao-Xia %A MA Shi-Rong %A CUI Yan %A WANG Gang %A
赵庆芳 %A 李巧峡 %A 马世荣 %A 崔燕 %A 王刚 %J 生态学报 %D 2006 %I %X Kobresia humilis is a very important fine pasture and ecological grass in the Qinghai-Tibet Platau. In present study, the genetic diversity of K.humilis collected from eastern of the Qinghai-Tibet plateau in China, were investigated using RAPD markers. 194 loci were identified with 14 oligonucleotide primers, out of which 168 loci were polymorphic. K.humilis showed high genetic diversity: The percentage polymorphic loci (PPB%) was 86.60%, Nei's gene diversity (h) was 0.2622 and Shannon's information index (I) was 0.3983 at the species level; while the PPB% was 62.65%, h was 0.2126 and I was 0.3185, at the level of population. Moreover, the results showed that there was a relationship between the habitats and genetic diversity of K.humilis, but no correlation between the altitude and genetic diversity. AMOVA showed that most of the genetic variability (83.04%) resided among individuals within populations, whereas only 16.96% resided among populations. The Gst-values (0.1891) showed the similar result. Additionally, Nei's unbiased genetic distance matrix compared with a corresponding geographic distance matrix showed the two matrices were not significantly correlated (r=0.37779,p=0.9718>0.05). %K RAPD
遗传多样性 %K 矮生嵩草 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=FE163E5DB2274E5937319DE98913EC37&aid=A284ED0B0077753D&yid=37904DC365DD7266&vid=96C778EE049EE47D&iid=5D311CA918CA9A03&sid=B69FEB12D9A4855D&eid=C6D7532EA8380B5C&journal_id=1000-0933&journal_name=生态学报&referenced_num=9&reference_num=43