%0 Journal Article
%T Molecular detection of symbiotic bacterium Buchera from aposymbiotic aphid Sitobion avenae<br>麦长管蚜脱巴克纳氏共生菌的分子验证
%A HU Zu-qing
%A KANG Ju-xia
%A ZHAO Hui-yan
%A HU Xiang-shun
%A <br>胡祖庆
%A 亢菊侠
%A 赵惠燕
%A 胡想顺
%J 生态学杂志
%D 2008
%I 
%X To detect the aposymbiotic effect of aphids is of significance in understanding the symbiotic system of aphids and Buchera. In this paper, the aposymbiotic effect of Sitobion avenae on symbiotic bacterium Buchera was studied by PCR technique combined with transmission microscope. The results showed that after treated S. avenae with rifampicin for 5- and 10-days, some expected fragments in Buchera bacteria were detected, suggesting that the symbionts in aphid body cavity were not injured. However, after treated with rifampicin for 15-days, the fragments disappeared, suggesting that the symbionts were completely disrupted. The observation with transmission microscopy was consistent with the diagnosis with PCR.
%K Sitobion avenae
%K Buchnera
%K aposymbiosis
%K PCR<br>麦长管蚜
%K 巴克纳氏菌
%K 脱共生
%K PCR
%K 麦长管蚜
%K 巴克纳氏菌
%K 共生菌
%K 分子验证
%K avenae
%K aphid
%K bacterium
%K detection
%K 降解
%K 核酸
%K 胞内共生细菌
%K 存在
%K 扩增条带
%K Buchnera
%K 发现
%K 检测
%K 处理
%K 利福平
%K 结果
%K 蚜虫脱共生效果
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=B04840A985D251F36078BD07A90EF003&aid=74236D6644AAA31740F7ABF49FEA0E4F&yid=67289AFF6305E306&vid=DB817633AA4F79B9&iid=CA4FD0336C81A37A&sid=6FBD78E3BAB60869&eid=28F8B56DB6BEE30E&journal_id=1000-4890&journal_name=生态学杂志&referenced_num=0&reference_num=15