%0 Journal Article
%T Genetic variability and genetic structure in clonal population of Arundinella hirta<br>野古草种群克隆的遗传变异和遗传结构
%A HONG Rui-Min
%A <br>洪锐民
%J 生态学报
%D 2004
%I 
%X Authors made approach to genetic variability and genetic structure of 30 clonal populations of Arundinella hirta in northeastern Song Nen steppe in China using enzyme electrophoresis and isozome analysis. Our study obtained the following results: means of P, A, AP, Ho and He in 30 populations were 17.00%, 1.17, 2.03, 0.103 and 0.063, respectively at the population level. The population with highest allelic diversity possessed 38.71% of the total allelic diversity in our sample. All of the alleles encountered in this study can be found in eight populations, including six with unique alleles and two others (RY3 and AS1). These populations appeared in all geographic areas. At the population level, average expected heterozygosity was 0.063. Observed heterozygosity 0.103 and ranging from 0.030 to 0.184. When genetic distances among all population pairs inhabiting the same large population and all pairs inhabiting the small population in the same region were compared, we found no significant relationship between genetic and geographic distances (P>0.56), and no significant difference between the two categories (P>0.89). Genetic diversity, including allelic richness, % polymorphic loci, obeserved heterozygosity, differed signifcantly between large population and small population (P>0.21). In 15 small populations, 57 of the 63 recorded alleles were found, in 15 large populations, 57 of the 63 alleles also were found. Levels of genetic variation did differ, however, among geographic areas within the study area (P>0.04). More than 40% genetic variation (G_(ST)=0.410) may be attributed to the variation among populations. Clonal reproduction and obligate outcrossing of Arundinella hirta complicated identification of other sources of variation. Observed levels of heterozygosity within populations were greater than have been expected if ramets were the products of random sexual reproduction. Values of total inbreedings, which compare observed heterozygosity within population with expected heterozygosity based on random mating within the entire study region. Higher Fst vallues for all loci indicated significant genetic differentiation at the population level, and the values ranged from 0.0890 to 0.8849. Populations were differentiated from one another within areas roughly to the same extent as they were differentiated within the entire study region. Calculations of gene flow indicated low levels of migration among populations of Arundinella hirta. Estimates of Nm<1 showed that gene flow between populations was inadequate to counter the effects of genetic drift in local populations. Measured values of genetic variation of Arundinella hirta population in this paper were more or less approximate to values estimated by Hamrick and Godi, Wolf et al. However, changes in genetic diversity of Arundinella hirta populations differed from other reports in genetic variations among populations, within populations in more or less extent. This variations occurred not only amo
%K clonal plant
%K genetic diversity
%K genetic structure<br>克隆植物
%K 遗传多样性
%K 遗传结构
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=FE163E5DB2274E5937319DE98913EC37&aid=D410E9B69A456649&yid=D0E58B75BFD8E51C&vid=B91E8C6D6FE990DB&iid=94C357A881DFC066&sid=51FE5F6F4794AD16&eid=8637B749179B02B3&journal_id=1000-0933&journal_name=生态学报&referenced_num=3&reference_num=26