%0 Journal Article
%T A modified method for measuring soil hydroxylamine reductase activity<br>土壤羟胺还原酶活性测定方法的改进
%A SHI Yun-feng
%A WU Zhi-jie
%A SHI Yi
%A CHEN Li-jun
%A WANG Shu-yi
%A <br>史云峰
%A 武志杰
%A 史奕
%A 陈利军
%A 王殳屹
%J 生态学杂志
%D 2007
%I 
%X In this paper,the traditional measuring method of soil hydroxylamine reductase activity was partially modified.The anaerobic condition needed for the measurement was created by a 3-5 cm fluid seal,which was made from the boiled water having been cooled down under airproof condition.The air above the fluid seal was exhausted with N2 current to prevent O2 dissolving into water.Compared with traditional methods,this method could reduce the uncertainty and the complexity in operation,and increase the maneuverability.Taking iodometric analysis as the reference,four spectrophotometric methods were adopted to determine the hydroxylamine concentration in extract,and the results showed that ammonium ferric sulfate-phenanthroline method was of high accuracy and precision,being feasible in measuring soil hydroxylamine reductase activity.
%K hydroxylamine reductase
%K anaerobic condition
%K spectrophotometry<br>羟胺还原酶
%K 厌氧环境
%K 分光光度法
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=B04840A985D251F36078BD07A90EF003&aid=125567494B44485E&yid=A732AF04DDA03BB3&vid=96C778EE049EE47D&iid=DF92D298D3FF1E6E&sid=6DAA05ECA4CD5075&eid=966760CE8B6E636C&journal_id=1000-4890&journal_name=生态学杂志&referenced_num=0&reference_num=13