%0 Journal Article
%T Quantification of the Frequency and Multiplicity of Infection of Respiratory- and Lymph Node¨CResident Dendritic Cells During Influenza Virus Infection
%A Rebecca VanOosten Anderson
%A Jodi McGill
%A Kevin L. Legge
%J PLOS ONE
%D 2012
%I Public Library of Science (PLoS)
%R 10.1371/journal.pone.0012902
%X Previous studies have demonstrated that DC differentially regulate influenza A virus (IAV)¨Cspecific CD8 T cell responses in vivo during high and low dose IAV infections. Furthermore, in vitro infection of DC with IAV at low versus high multiplicities of infection (MOI) results in altered cytokine production and a reduced ability to prime na£¿ve CD8 T cell responses. Flow cytometric detection of IAV proteins within DC, a commonly used method for detection of cellular IAV infection, does not distinguish between the direct infection of these cells or their uptake of viral proteins from dying epithelial cells.
%U http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0012902