%0 Journal Article
%T Microbial diversity in fluidized pellet-bed bioreactor<br>生物造粒流化床污水处理反应器的微生物多样性
%A YUAN Hong-lin
%A LIU Yong-jun
%A WANG Xiao-chang
%A MA Mei-ling
%A ZUO Li-li
%A <br>袁宏林
%A 刘永军
%A 王晓昌
%A 马美玲
%A 左丽丽
%J 生态学杂志
%D 2007
%I 
%X To study the microbial diversity of granular sludge in fluidized pellet-bed (FPB) bioreactor, the sludge at different heights (10, 60 and 110 cm) of FPB was sampled, and the genomic DNA of microbial community was extracted directly. After purifying the genomic DNA, 16S rRNA genes were amplified, and the amplified DNA fragments were separated by denaturing gradient gel electrophoresis (DGGE). The characteristic bands of DGGE were sequenced, and the phylogenetic diversity of microbes in the FPB bioreactor was surveyed based on the homology analysis of 16S rRNA sequences. The results indicated that the obtained 18 operational taxonomic units (OTUs) all belonged to Eubacteria, of which, 61% was Proteobacteria, 17% was Actinobacteria, 11% was low G C gram-positive bacterium, and 11% belonged to other bacterial branches.
%K 16S rRNA<br>变性梯度凝胶电泳
%K 生物造粒流化床反应器
%K 微生物多样性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=B04840A985D251F36078BD07A90EF003&aid=9B1BD99BEF4672DE&yid=A732AF04DDA03BB3&vid=96C778EE049EE47D&iid=5D311CA918CA9A03&sid=EB3DE2AA673AE431&eid=9E33BE6F309BC209&journal_id=1000-4890&journal_name=生态学杂志&referenced_num=0&reference_num=14