%0 Journal Article
%T EFFECTS OF SOYBEAN OLIGOSACCHARIDES ON NUTRITIONAL CHARACTERS OF JAPANESE FLOUNDER (PARALICHTHYS OLIVACEUS): Ⅱ. DIGESTIBILITY AND DIGESTIVE PHYSIOLOGY<br>大豆低聚糖对牙鲆营养效应的研究：Ⅱ 消化率和消化生理
%A DENG Jun-Ming
%A MAI Kang-Sen
%A AI Qing-Hui
%A ZHANG Wen-Bing
%A <br>邓君明
%A 麦康森
%A 艾庆辉
%A 张文兵
%J 水生生物学报
%D 2009
%I 
%X A feeding trial was conducted to investigate the effects of soybean oligosaccharides (SBOS) on the digestive enzymes activity, feed digestibility and intestinal morphology in juvenile Japanese flounder (Paralichthys olivaceus). Four isonitrogenous and isocaloric practical diets (crude protein 49%, gross energy 19 kJ/g) including only fish meal (FM) or soy protein isolate (SPI) as a sole protein source with (Diets FMO and SPIO) or without (Diets FM and SPI) supplemented SBOS (stachyose, 2.61%; raffinose, 0.61%) were formulated. Each diet was randomly assigned to triplicate aquaria stocked with 30 fish (initial body weight 1.93 ± 0.02 g) each. Fish were maintained in flow-through aquaria at water temperature ranging from 22.0 °C to 25.0 °C and fed twice (08:00 and 16:00) daily to apparent satiation for 8 weeks. Pepsin/protease activities in alimentary tract and liver were generally decreased by supplemental SBOS, but the difference was not significant (p < 0.05). Dietary SBOS supplementation significantly decreased the lipase activity in liver (p < 0.05), but not the alimentary tract in fish fed FM-based diets. Supplemental SBOS significantly increased the lipase activity in foregut, decreased in stomach and midgut (p < 0.05), but did not affect in pylorus, hindgut and liver in fish fed SPI-based diets (p > 0.05). Supplementation with SBOS significantly decreased the amylase activity in midgut, increased in stomach (p < 0.05), but did not affect in pylorus, foregut, hindgut and liver in fish fed FM-based diets (p > 0.05). Supplemental SBOS significantly increased the amylase activity in stomach and foregut, decreased in midgut (p < 0.05), but did not affect in pylorus, hindgut and liver in fish fed SPI-based diets (p > 0.05). At the same time, dietary SBOS supplementation significantly decreased the apparent digestibility coefficients (ADC) of dry matter, increased the crude lipid and energy (p < 0.05), but did not affect the crude protein of Diet FM (p > 0.05). ADCs of dry matter, crude protein, crude lipid and energy of Diet SPI were not significantly affected by dietary supplemental SBOS (p > 0.05). Intestinal morphology was not obviously influenced by dietary supplemental SBOS in fish fed FM or SPI-based diets. These results indicated that dietary supplemental SBOS did not negatively affect the digestion of Japanese flounder and SBOS was not the main factor which affected the utilization of soy protein sources.
%K Japanese flounder (Paralichthys olivaceus)
%K Soybean oligosaecharides
%K Nutritional characters
%K Digestibility
%K Digestive physiology<br>牙鲆
%K 大豆低聚糖
%K 营养效应
%K 消化率
%K 消化生理
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=256651267D5D2E4520A7707D05E98747&aid=7AE9FEE657046769299DAF5FE013C3C5&yid=DE12191FBD62783C&vid=27746BCEEE58E9DC&iid=38B194292C032A66&sid=CA4FD0336C81A37A&journal_id=1000-3207&journal_name=水生生物学报&referenced_num=1&reference_num=23