%0 Journal Article
%T SIUDIES ON THE SUPEROXIDE-GENERATING ACTIVITY OF NADPH OXIDASE BY CHEM1LUMINESCENCE<br>用化学发光法研究NADPH氧化酶产生O_2~+的活性
%A 杨法军
%A 赵保路
%A 忻文娟
%J 生物物理学报
%D 1991
%I 
%X The respiratory burst oxidase(or NADPH oxidase)is a membrane-bound enzyme which is responsible for the production of superoxide anions by phagocytes. The activity of the NADPH oxidase from PMA-stimulated and control human PMN in a fully soluble cell-free system was studied by luminol-dependent chemilumine-scence(CL). The CL value increased with the NADPH concentration, and increased linearly with the concentration of the enzyme. The activity of the NADPH oxidase of PMA-stimulated PMN was much higher than that of the control PMN at the same concentration of NADPH. The time-course of CL value was presented. In addition, the rate of superoxide anion production determined by the superoxide-dismutasc (SOD) sensitive ferricytochrome-C reduction was studied in comparison. We presume that the process of PMA stimulation is probably an assembly of the components of the NADPH oxidase.
%K 多形核白细胞
%K NADPH氧化酶
%K O^－O
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=5E86E445AE6962DC61DD98C2ADBCB032&yid=116CB34717B0B183&vid=DF92D298D3FF1E6E&iid=E158A972A605785F&sid=BB98BB04E861B6F5&eid=D45762219109E903&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=4&reference_num=2