%0 Journal Article
%T CHARACTERISTICS OF EMODIN EVOKED i ANDINHIBITION OF GDP IN GUINEA PIG TAENIA COLI CELLS<br>大黄素升高豚鼠结肠带细胞[Ca~(2+)]_i的特征和GDP的抑制作用
%A YNAG Wen-xiu
%A <br>杨文修
%A 王津
%A 李俊英
%A 余奕
%A 许文胜
%J 生物物理学报
%D 2001
%I 
%X Intracellular free Ca2+ concentration (i) was detected using Fluo-3 fluorescence probe. Relationship between emodin concentration and intracellular i as well as kinetics of i in guinea pig taenia coli cells were studied. In the range of lower emodin concentration,i rose remarkably with increasing of drug concentration. Supermaximal stimulation of emodin inhibited rising of i. Inhibition of GDP on rising of i enhanced with increasing of GDP concentration. Effects of GDP and extracellular Ca2+- free on rising kinetics of emodin evoked i were studied. Peak of i disappeared by adding GDP. When extracellular Ca2+-free, i decreased remarkably with prolonging of time, rising of emodin evoked i tended to disappear. These results suggest that emodin could evoke Ca2+ release from intracellular Ca2+ pool by activating receptor coupled G protein and promote extracellular Ca2+ influx by depolarizing cell membrane potential.
%K Emodin
%K Guinea pig taenia coli cells
%K i
%K GDP
%K Kinetics<br>大黄素
%K 豚鼠结肠带细胞
%K GDP
%K 动力学
%K 钙离子
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=111707A8792891D3&yid=14E7EF987E4155E6&vid=BCA2697F357F2001&iid=CA4FD0336C81A37A&sid=8BD23BD67BF01A5C&eid=CB423C9A71560A74&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=1&reference_num=12