%0 Journal Article
%T COMPARATIVE STUDIES ON THE ENZYMOLOGICAL CHARACTERISTICS OF SNase R AND SNase<br>SNase R与SNase酶学性质的比较研究
%A Zhang Guangfa Jing Guozhong
%A <br>张广发
%A 静国忠
%J 生物物理学报
%D 1993
%I 
%X SNase R, an analogue of staphylococcal nuclease (SNase) , which has additional six amino residues at its amino -terminus, was over-produced by recombinant DNA manipulation and purified by ion exchange chromatography on a phosphocellulose column. The purified enzyme gave a single band with a molecular weight of 17. 5KDa on SDS - polyacrylamide gel. The comparative studies of SNase R and SNase showed that the additional six amino acid residues at the N - terminus did not affect the Kinetic properties of the enzyme. The studies also provided an essential basis for using SNase R as a material for studying relationship between structure and function of protein, as well as polypeptide folding.
%K Staphylococcal Nuclease Kinetic Analysis Protein Purification<br>酶学
%K 金黄葡萄球菌
%K 核酸酶
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=EE30774F17A913B1D1CA4D2BE65D7B94&yid=D418FDC97F7C2EBA&vid=9CF7A0430CBB2DFD&iid=CA4FD0336C81A37A&sid=96C778EE049EE47D&eid=4AD960B5AD2D111A&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=2&reference_num=1