%0 Journal Article
%T Diallyl Disulfide-induced Apoptosis of Raji cells is Mediated by Caspase Activation and Cytochrome c Release<br>二烯丙基二硫通过激活Caspase3和释放Cyt-c诱导Raji细胞凋亡
%A 谭晖
%A 吉晓霞
%A 凌晖
%A 罗招阳
%A 何洁
%A 易岚
%A 周建国
%A 苏琦
%J 生物物理学报
%D 2009
%I 
%X Diallyl disulfide (DADS) is a natural organosulfur compound derived from crushed garlic, it has been reported that the antiproliferative activity of DADS in several cancer cell lines. However, the molecular mechanism underlying the DADS-antitumor effect is not yet clearly understood. In the present study, the CCK-8 was used by assay the inhibition of DADS on the cells viability, the DADS-induced apoptosis in human Raji cells were investigated using both morphological analysis and DNA fragmentation technique. In this study, demonstrated that DADS-induced apoptosis in human Raji cells is a concentration- and time-dependent manner. This DADS-induced apoptosis in human Raji cells was also accompanied by the down-regulation of Bcl-2 and MCL-1 and activation of Bid, but no effect on the levels of Bax and Bak. Moreover, the DADS treatment resulted in increase in the release of mitochondrial cytochrome c (cyt c), and activation of caspase 3. Furthermore, treatment with caspase  inhibitor (z-DEVD-fmk) was capable of preventing the DADS-induced caspase 3 activity and cell death. These results clearly demonstrate that the induction of apoptosis by DADS involves be mediated, at least in part, by the Bcl-2/MCL1-mitochondria-Caspase3 pathway in human Raji cells.
%K Diallyl disulfide
%K Raji cells
%K Apoptosis
%K Caspase3<br>DADS
%K Raji细胞
%K 凋亡
%K Caspase3
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=993BF1162C88B1CA1E98C5F362CA4A58&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=CA4FD0336C81A37A&sid=BC12EA701C895178&eid=D3E34374A0D77D7F&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=0&reference_num=0