%0 Journal Article
%T STUDY ON THE MEMBRANE INSERTION DEPTH AND ORIENTATION OFHIV-1 FUSION PEPTIDE BY FOURIER TRANSFORMEDINFRARED SPECTORSCOPY<br>用傅里叶变换红外光谱技术研究HIV-1融合肽的插膜深度与角度
%A QIU Yang
%A LIN Ke-chun
%A NIE Song-qing
%A <br>邱阳
%A 林克椿
%A 聂松青
%J 生物物理学报
%D 2001
%I 
%X To investigate the mechanism of membrane fusion induced by HIV-1 gp41 N terminal fusion peptide, we studied its membrane insertion state and orientation by H/D exchange and polarized FTIR in acidic POPG and neutral POPC liposomes, respectively. The results indicate that only 11.9% of backbone amide protons of HIVWT in POPG vesicles undergo H/D exchange, whereas 44.6% amide protons are exchangeable for HIVWT in POPC SUV. The average angles of α-helix and β-strand of HIVWT with respect to the POPG bilayer surface are 29±2° and 25±1°, respectively. In the presence of POPC SUV, the average angle between the α-helix of HIVWT and the bilayer surface is 22±1°, and the average angle of β-strand of HIVWT is 26±3°. The membrane insertion state of HIVWT was discussed.
%K HIV-1 fusion peptide
%K FTIR
%K H/D exchange
%K Orientation<br>HIV－1融合肽
%K FTIR
%K H／D交换
%K 分子取向
%K 人类免疫缺陷病毒Ⅰ型
%K 插膜深度
%K 傅里叶变换红外光谱
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=E0C9D9BBED813D6674AC13E942EAC86D&aid=2AB5DB4DD9C68A8B&yid=14E7EF987E4155E6&vid=BCA2697F357F2001&iid=CA4FD0336C81A37A&sid=D997634CFE9B6321&eid=286FB2D22CF8D013&journal_id=1000-6737&journal_name=生物物理学报&referenced_num=2&reference_num=14