%0 Journal Article
%T CLONING AND EXPRESSION OF WHITE CLOUD MOUNTAIN MINNOW (TANICHTHYS ALBONUBES) CASPASE-3 AND CASPASE-9 cDNAs<br>唐鱼Caspase-3和Caspase-9 cDNA全长克隆及胁迫表达分析
%A XU Sheng-Wei
%A LI Tian
%A WU Jin-Ying
%A LIANG Jian-Hong
%A LIU Han-Sheng
%A LIAN Chang-Ping
%A <br>徐胜威
%A 李恬
%A 吴金英
%A 梁健宏
%A 刘汉生
%A 连常平
%J 水生生物学报
%D 2011
%I 
%X The white cloud mountain minnow (Tanichthys albonubes) is an important aquarium fish, and also a potential experimental model organism. In order to get the knowledge of apoptosis under environmental stress in this species, we have firstly cloned and sequenced the full-length cDNAs of Caspase-3 and Caspase-9 from the gill tissue of T. albo-nubes using homology cloning approach combined with RACE method. In this study, the bioinformatics analysis of Caspase-3 and Capase-9 full-length cDNA sequences were presented. The results indicated that the full-length cDNA of Caspase-3 was composed of 2153 base pairs (bp), which included a 840 bp open reading frame (ORF) encoding a 279-amino acid peptide, whereas the full-length cDNA of Caspase-9 was composed of 1758 bp, which included a 1323 bp ORF encoding a 440-amino acid peptide. The identity comparison and the phylogenetic analysis showed that the predicted Caspase-3 and Caspase-9 proteins of T. albonubes were structurally similar to those of other fish, with high conservation in important functional domains. Real-time PCR analysis showed that the expression levels of Caspase-3 and Caspase-9, under cypermethrin-induced stress conditions, were suppressed compared to control group, and that Caspase-3 and Caspase-9 mRNA levels were significantly affected in a dose- and time-dependent manner by cyperme-thrin. These results suggest that cypermethrin plays a role in the suppression of apoptosis of T. albonubes, and that the anti-apoptotic effect of cypermethrin may be beneficial to this fish so that it can survive in harsh environments.
%K Tanichthys altbonubes
%K Caspase-3
%K Caspase-9
%K Cloning
%K Cypermethrin
%K Expression<br>唐鱼
%K Caspase-3
%K Caspase-9
%K 克隆
%K 氯氰菊酯
%K 表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=256651267D5D2E4520A7707D05E98747&aid=2B40DEE7D69633794E95F44DC8E24880&yid=9377ED8094509821&vid=6209D9E8050195F5&iid=CA4FD0336C81A37A&sid=09E495F616948E78&eid=3986B25773CB6C30&journal_id=1000-3207&journal_name=水生生物学报&referenced_num=0&reference_num=26